Your benchmark for fluorescent protein flow cytometry
Flow cytometry with the MACSQuant Family is defined by performance, automation, compactness, and convenience. Accordingly, the MACSQuant VYB offers a comprehensive suite of automation features for start‑up, maintenance, acquisition, analysis, and shutdown. It also features a uniquely configured optical bench with violet, yellow, and blue lasers. This makes the MACSQuant VYB a perfect fit for detecting fluorescent protein reporters and provides optimized separation of PE and FITC conjugates – ensuring consistent, high‑quality results.
Full automation
Load your samples, import your experiment settings, and leave the staining, data acquisition, and analysis to the MACSQuant VYB.
Fluorescent protein analysis
Fully utilize fluorescent proteins and reporter applications that require the violet, blue, or yellow laser, such as mCherry, GFP, and CFP.
Flexible assay setup
Easily and immediately switch between tubes and plates at the click of a button.
Multi-instrument alignment
Harmonize data with collaborating labs to ensure reproducibility.
MACSQuant VYB specifications
| Optics | |
| Laser excitation | Spatially separated: 405 nm, 40 mW 488 nm, 50 mW 561 nm, 100 mW |
| Emission detectors | FSC: 561/4 nm SSC: 561/4 nm V1: 452/45 nm V2: 525/50 nm Y1: 586/15 nm Y2: 615/20 nm Y3: 661/20 nm Y4: 740 nm LP B1: 525/50 nm B2: 593–650 nm |
| Flow cell dimensions | 200 × 250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
| Fluidics | |
| Minimal uptake volume | 1 μL (25 μL recommended for volumetric counting applications) |
| Sample flow rate | 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second |
| Measurement speed | <25 minutes per 96-well plate (5 μL measurement volume per well) |
| Sample uptake | 1–450 µL |
| Maximal event rate | Up to 15,000 events/second |
| Technical specifications | |
| Footprint | 385.5 × 284.5 mm (15.18×11.2 in) |
| Size (width×depth) | 669 × 400 mm (26.34×15.75 in) |
| Height (adjustable touchscreen) | 394–553.5 mm (15.51–21.79 in) |
| Size with miniSampler Plus (width×depth) | 669 × 500 mm (26.34×19.69 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6″ LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display), 2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
| Performance | |
| Sample carry-over | 0.01% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
| Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 8×8 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
