The UltraMicroscope Blaze is a user-friendly light sheet microscope designed for subcellular 3D imaging of large and multiple samples.
As the successor to the first commercial light sheet microscope, it has been developed based on decades of customer feedback and sets a benchmark with cutting-edge technology.
The new LightSpeed Mode ensures exceptional data quality at unprecedented speeds. A standard sample chamber accommodates multiple rodent organs/organoids, while the XXL upgrade expands capacity for human kidney or whole adult mouse models. MACS iQ View-3D Large Volume, a software package for image processing, maximizes the potential of your data.
Automated features
The UltraMicroscope Blaze enables an automated sample release and smooth switching between different objectives and magnification lenses, while keeping images sharp with the autofocus feature.
Large sample chambers
A standard sample chamber allows convenient imaging of multiple rodent organs or organoids, and the new XXL chamber provides the largest possible sample space for the UltraMicroscope Blaze.
Easy adaptation
Whether imaging large organs or even entire mouse models, our wide range of dipping caps guarantees high image quality in all clearing media – from water to organic solvents.
High-resolution
The combination of the MI Plan objective lenses and the cutting-edge light sheet illumination technology ensures unprecedented image quality from overview to sub-cellular details.
UltraMicroscope Blaze specifications
| Sheet optics | |||
| Illumination | Uni- and bi-directional | ||
| Number of light sheet | 1–6 | ||
| Thickness | 4–24 µm | ||
| Width | 1–20 mm | ||
| Numerical aperture | 0.0135–0.135 | ||
| Focus positioning | Dynamic | ||
| Detection optics | |||
| Objective lenses | 1.1x | 4x | 12x |
| Total magnification | 0.66–2.75× | 2.4–10× | 7.2–30× |
| Numerical aperture | 0.1 | 0.35 | 0.53 |
| Max. theoretical resolution at detector | 4.8 µm | 1.3 µm | 0.5 µm |
| Working distance | ≤17 mm | ≤16 mm | ≤10.9 mm |
| FOV diagonal | 6.8–29 mm | 1.9–7.8 mm | 0.63–2.6 mm |
| Emission filters | Seven filters ⌀ 43 mm | ||
| Chromatic correction | Dynamic 400–850 nm | ||
| Sample chamber | |||
| Chamber name | Cuvette for UltraMicroscope Blaze | ||
| (standard sample chamber) | XXL chamber for UltraMicroscope Blaze | ||
| Capacity | 497 mL | 715 mL | |
| Inner dimensions (w × d × h) | 5.0 cm × 18.3 cm × 6.4 cm | 7.1 cm × 20.0 cm × 6.4 cm | |
| Max. imaging range (x, y, z) | 3.5 cm, 6.0 cm, 1.7 cm | 4.4 cm, 7.0 cm, 1.7 cm | |
| Max. imaging volume | 35 cm3 | 53 cm3 (+51%) | |
| Imaging solution | Aqueous buffers and organic solvents | ||
| Batch imaging mode | Multiple samples can be imaged in one session. | ||
| Refractive index compatibility | 1.33–1.56 | ||
| General information | |||
| Dimensions (w × h × d) | 67 cm × 91 cm ×52.5 cm | ||
| Weight | 98 kg (w/o controller and laser) | ||
| Light sources | |||
| Laser beam combiner | Max. 5 laser lines (405, 488, 561, 639, 785 nm)*, 50 mW–150 mW per diode | ||
| Supercontinuum white light laser | 405–850 nm spectral range, 1–4 mW/nm | ||
| Camera specifications | |||
| Detector | 4.2 Megapixel sCMOS camera | ||
| Active pixels (w × h) | 2048 × 2048 | ||
| Pixel size | 6.5 µm × 6.5 µm | ||
| Sensor size | 13.3 mm × 13.3 mm; 18.8 mm diagonal | ||
| Readout noise | 0.8 med e- | ||
| Maximal frame rate | 100 fps | ||
| Maximum quantum efficiency | 82% | ||
